Journal: iScience

Article Title: Circadian disruption exacerbates MASH by reducing Akkermansia muciniphila via the FXR-CYP7A1-bile acid axis

doi: 10.1016/j.isci.2026.115397

Figure Lengend Snippet: Circadian disruption promotes inflammation, lipid accumulation, and fibrosis in MASH mice (A) The relative mRNA expression of lipid-metabolism-related genes ( Srebp , Fasn , and Acaca ), inflammation-related genes ( Cxcl2 , Ccl2 , and Nos2 ), and fibrosis-related genes ( Col3a1 , Col4a1 , and α-Sma ) in MASH with or without circadian disruption, n = 6. (B) Flow cytometry analysis of the abundance of macrophages (CD11b + F4/80 + ), M1 cells (CD86 + CD206 − ), and M2 cells (CD86 − CD206 + ) in the liver in each group, n = 6. (C) Comparison of M1/M2 polarization in the liver in each group by CD86/CD206 detected by immunohistochemistry. Scale bars: 100 μm (10×). (D) Serum IL-6, IL-1β, and TNF-α in each group, n = 6. (E) The relative expression level of the oxidative stress indicator ROS, the content of GSH, and the relative mRNA expression level of Hif-1α related to them in each group, n = 6. (F) The total number of genes detected by liver transcriptomics, as well as the number of genes that are relatively upregulated and downregulated in the two groups. (G) NMDS plot of the main components of liver transcriptomics in each group, n = 4. (H) Reactome analysis results (barplot) of liver transcriptomics in the two groups. (I) Reactome analysis results (dotplot) of liver transcriptomics in the two groups. Data are expressed as mean ± SD ( n = 4–6), ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, compared with the MASH group. p values were calculated using unpaired t test. Srebp , sterol regulatory element-binding protein; Fasn , fatty acid synthase; Acaca , acetyl-CoA carboxylase; Cxcl2 , chemokine (C–X–C motif) ligand 2; Ccl2 , chemokine (C–C motif) ligand 2; Nos2 , nitric oxide synthase 2; Col3a1 , collagen alpha-1 (III) chain; Col4a1 , collagen alpha-1 (IV) chain; α-Sma , α-smooth muscle actin; M1, M1 polarization of macrophages; M2, M2 polarization of macrophages; CD11b, marker of mouse macrophages; F4/80, marker of mouse macrophages; CD86, marker of M1 polarization of mouse macrophages; CD206, marker of M2 polarization of mouse macrophages; IL-6, interleukin-6; IL-1β, interleukin-1 beta; TNF-α, tumor necrosis factor-alpha; ROS, reactive oxygen species; GSH, glutathione; Hif-1α , hypoxia inducible factor-1 alpha; NMDS, non-metric multidimensional scaling.

Article Snippet: Rabbit anti-CD206 antibody , Cell Signaling Technology , Cat# 24595; RRID: AB_2892682.

Techniques: Disruption, Expressing, Flow Cytometry, Comparison, Immunohistochemistry, Transcriptomics, Binding Assay, Marker

Journal: Materials Today Bio

Article Title: Ultrasound-activated piezoelectric Silk-PVDF hydrogel reprograms the osteoimmune microenvironment via NRF2 signaling for accelerated bone regeneration

doi: 10.1016/j.mtbio.2026.102779

Figure Lengend Snippet: Piezoelectric hydrogel activates NRF2 to attenuate ROS and macrophages polarization for osteogenesis . (A–B) RT-qPCR results for the mRNA expression of pro-inflammatory differentiation of macrophages. (C–D) RT-qPCR results for the mRNA expression of anti-inflammatory differentiation of macrophages. (E) The relative protein expression levels of INOS, CD206. (F–G) Semi-quantitative analysis of immunoblotting results of INOS, CD206. (H) ROS staining of ADSCs. (I) Mean intensity of ROS staining. (J) The relative protein expression levels of NRF2, NQO1, GPX4. (K–M) Semi-quantitative analysis of immunoblotting results of NRF2, NQO1, GPX4. Data are presented as the mean ± SEM; n = 3; ∗significant difference between selected groups, ∗ p < 0.05, ∗∗ p < 0.01 and ∗∗∗ p < 0.001.

Article Snippet: Cells were fixed with 4 % paraformaldehyde (Servicebio, #G1101) for 15 min at 25 °C, permeabilized with 0.3 % Triton X-100 in PBS for 15 min, and blocked with 5 % BSA (Sigma, #A7906) containing 10 % normal goat serum (Servicebio, #G5009) for 1 h. Primary antibodies were diluted in antibody diluent (Servicebio, #G1212) and incubated overnight at 4 °C: • Osteopontin (OPN): Rabbit monoclonal (Proteintech, #22952-1-AP), 1:500 • Osteocalcin (OCN): Rabbit polyclonal (Proteintech, #20277-1-AP), 1:500 • iNOS: Mouse anti-iNOS (Proteintech, #22226-1-AP), 1:500 • CD206: Rabbit anti-CD206 (Proteintech, #18704-1-AP), 1:500 After three PBS washes, species-matched secondary antibodies were applied for 1 h at 25 °C in the dark: • Alexa Fluor 488: Goat anti-rabbit IgG (Servicebio, #GB25303), 1:500 • Alexa Fluor 488: Goat anti-mouse IgG (Servicebio, #GB25301), 1:500 • Cy3: Goat anti-rabbit IgG (Servicebio, # GB21303), 1:500 Nuclei were counterstained with DAPI (Servicebio, #G1407).

Techniques: Quantitative RT-PCR, Expressing, Western Blot, Staining

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Targeting FAM83D triggers tumor cell senescence via cGAS-STING signaling activation and reprograms TAMs to combat glioma

doi: 10.1186/s13046-026-03681-y

Figure Lengend Snippet: Knockdown of FAM83D leads to the infiltration of M1 TAMs. A Expression of genes associated with chemotaxis, IL1 production, IL6 production, and IFN-γ production in RNA-seq of the U251 cell line. B UMAP plot of TAMs from scRNA-seq colored by sub-clusters. C Expression of M1 markers ( Cd86 , Ccl3 , and Tnf ) and M2 markers ( Mrc1 , Arg1 , and Tgfbi ) in TAMs from scRNA-seq. D GO biological process enrichment based on the corresponding marker genes in each TAMs sub-cluster. p adjusted value, a corrected p value determined by bonferroni. E Proportion of each TAM’s sub-cluster across samples, respectively. p value, Chi-square test. F Enrichment scores of M1/M2 macrophage associated signatures in TAMs across samples. p value, two-sided unpaired Wilcoxon test. G Spatial structure model of glioma based on spatial transcriptomics. H Representative sample UKF266 showing the expression of FAM83D in each layer (left) and quantification of FAM83D positive spots in each layer (right). p value, two-sided unpaired t-test; error bars, mean ± SEM. I Representative samples UKF269 and UKF275 showing the relationship between the expression of FAM83D and the proportion of inflammatory macrophage (left) and their correlation analysis (right). p value, Pearson’s correlation test. J Representative HE staining image and staining image of Iba1, iNOS, and Cd206 in mouse brains under different treatments (top) and quantification of protein level normalized to the control group (bottom). Each data point represents one mouse ( n = 3 mice for each treatment). p value, two-sided unpaired t-test; error bars, mean ± SEM. K Schematic of cellular experiments verifying the effect of SASP induced by knockdown of FAM83D on macrophage polarization. L Relative expression of M1 markers ( Tnf , Il1b , and Cd86 ) and M2 markers ( Tgfb, Il10 , and Cd206 ) in the BV2 cell line treated with different conditioned medium. Each data point represents one biological replicate ( n = 3 replicates for each treatment). The culture time is 48 h. p value, two-sided unpaired t-test; error bars, mean ± SEM. M Representative staining image of Cd86 and Cd206 in the BV2 cell line co-cultured with tumor cells under different conditions (left) and quantification of protein level normalized to the control group (right). Each data point represents one random region of interest ( n = 10 random regions for each condition). The culture time is 48 h. p value, two-sided unpaired t-test; error bars, mean ± SEM

Article Snippet: Cells were incubated with rabbit anti-FAM83D antibody (Abcam, no. ab236882, 1:100), mouse anti-Ki67 antibody (Cell Signaling Technology, no. 9449 S, 1:1000), rabbit anti-γH2AX antibody (Cell Signaling Technology, no. 9718 S, 1:500), mouse anti-α-Tubulin antibody (Cell Signaling Technology, no. 3873 S, 1:1000), mouse anti-dsDNA antibody (Santa Cruz Biotechnology, no. sc-58749, 1:50), rabbit anti-STING antibody (Proteintech, no. 19851-1-AP, 1:100), rabbit anti-Cd206 antibody (Proteintech, no. 18704-1-AP, 1:500) or rabbit anti-Cd86 antibody (Abclonal, no. A1199, 1:100) overnight at 4 °C.

Techniques: Knockdown, Expressing, Chemotaxis Assay, RNA Sequencing, Marker, Spatial Transcriptomics, Staining, Control, Cell Culture